In the present study, we investigated the cellular effects caused by metal nanoparticlesĪnd nanocarbons, including gold (Au), silver (Ag), platinum (Pt), and hydrophilic carbon black (hCB). Therefore, understanding the toxicity of nanoparticles in their industrial forms is imperative.Īmong the nanoparticles, metal nanoparticles and nanocarbons are widely used. In addition, metal ions released from nanoparticles have been correlated to their cytotoxic activity For instance, nanoparticles exhibit high solubility compared to their micro-scale counterparts (Horie etĪl., 2009b). Properties also have biological influences that can be Nanoparticles have unique physical and chemical properties that are beneficial to industrial use. Currently, various kinds of nanoparticles are employed in many industries, including consumer products and the life sciences. Key words: Nanoparticle, Gold, Silver, Carbon black, Oxidative stressĪ nanoparticle is defined as a particle whose diameter Hence, the cellular effects of industrial nanoparticles should be evaluated carefully. In some cases, the dispersing agent may have caused some cellular effects.Īdsorption of agents on the surface of the nanoparticles may be an important factor here. Some nanoparticle dispersions included chemicals as the dispersant, which is The Pt nanopa rticles in this study contradicted our previous findings, in which Pt did not produce chemically reactive molecules. The observed increase in the ROS level induced by Hydrophilic carbon black did not exhibit any effects. The exposure to Pt nanoparticles inducedĪn increase in the intracellular reactive oxygen species (ROS) level. The exposure to Au and Ag decreased mitochondrial activity. The mitochondrial activity (MTT assay) and the induction of cellular oxidative stress were examined.
Medium of human keratinocyte (HaCaT) and human lung carcinoma (A549) cells for 6 and 24 hr. Stable nanoparticle dispersions were prepared and applied to the culture (Received AugAccepted October 28, 2014)ĪBSTRACT - The effects of five types of metal nanoparticles, gold (Au), silver (Ag), platinum (Pt),Īu-polyvinylpyrrolidone (PVP) colloid, and Pt-PVP colloid, and two types of hydrophilic carbon black Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST),Ģ217-14 Hayashi-Cho, Takamatsu, Kagawa 761-0395, JapanĢNational Metrology Institute of Japan, AIST, 1-1-1, Higashi, Tsukuba, Ibaraki 305-8565, JapanģTechnology Research Association for Single Wall Carbon Nanotubes (TASC), AIST Tsukuba West,ġ6-1, Onogawa, Tsukuba, Ibaraki 305-8569, JapanĤResearch Institute of Science for Safety and Sustainability (RISS), AIST, 16-1, Onogawa, Tsukuba, Ibaraki 305-8569, JapanĥResearch Institute of Instrumentation Frontier (RIIF), AIST, 1-1-1 Umezono, Ibaraki 305-8568, JapanĦFaculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan Kazuhiro Yamamoto5, Shinichi Kinugasa2, Yoshihisa Hagihara1, Yasukazu Yoshida1 Lilian Kaede Komaba1, Keiko Nishio1, Ayako Nakamura2, Arisa Miyauchi3, Masanori Horie1, Haruhisa Kato2, Shigehisa Endoh3, Katsuhide Fujita4, Sci.)Ĭellular effects of industrial metal nanoparticles and Pokimane lived through this exact scenario, and while not as dangerous as accidentally sharing credit card information, it is embarrassing nonetheless.The Journal of Toxicological Sciences (J. But when you come back, you realize the stream was displaying your personal email for the past minute for everyone to see. Before you start, however, you grab a bottle of water - a hydrated streamer is an alert streamer, after all. Imagine, if you will, playing Call of Duty: Warzone for the first time and celebrating the momentous occasion with a livestream.
However, this wasn't her only brush with personal info leaks. Pokimane recovered from this disastrous event quickly thanks to fast-acting card customer service. Something like that happened to Pokimane when her credit card was accidentally caught on stream by fellow creator Fedmyster, who was wearing a chest camera. Then, when you get home, you discover someone tried to use your card to purchase 50 pounds of Cheez Whiz. You're out on the town, minding your own business when your credit card is denied.
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